Recognition of whole Cryptosporidium oocysts in feces by negative staining and electron microscopy

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Recognition of whole Cryptosporidium oocysts in feces by negative staining and electron microscopy.

Crytosporidium oocysts in feces were recognizable in the electron microscope when prepared by the negative staining technique used by virologists. Their size, shape, and surface markings were sufficiently characteristic for cryptosporidiosis to be diagnosed by this method if more suitable methods are not available.

متن کامل

Negative staining of whole cells: transmission electron microscopy of peripheral organelles in rat venous endothelial cells.

The study of whole negatively stained cells has revealed details of cellular organelles in rat venous endothelial cells. In particular, details of surface membrane organelles and small tubular structures were demonstrated. The surface membrane organelles which appeared "vesicular-like" were found to be connected with small tubular attachments. These findings were correlated with those described...

متن کامل

Differentiation between Viable and Dead Cryptosporidium Oocysts Using Fluorochrome Staining.

The use of nucleic acid staining with a fluorochrome dye to differentiate viable and dead (heat-killed) Cryptosporidium oocysts was assessed. The specificities (percentage of unstained viable oocysts) and sensitivities (percentage of stained dead oocysts) of the seven tested dyes (SYTO-17® and SYTO-59® to 64®) ranged from 65 to 76% (average 71%) and 83 to 95% (average 91%), respectively. SYTO-5...

متن کامل

Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay.

A monoclonal antibody enzyme-linked immunosorbent assay (ELISA) was developed to detect Cryptosporidium parvum oocysts in bovine feces. Fecal oocysts were concentrated by centrifugation through Formalin-ethyl acetate solution and captured with monoclonal antibody 18.280.2 reactive with C. parvum oocysts. Captured oocysts were detected with goat anti-oocyst serum, following the addition of a per...

متن کامل

Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient.

Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1984

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.19.4.566-567.1984